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High-level description

The cassiopeia directory contains a comprehensive suite of tools and modules for phylogenetic analysis, particularly focused on single-cell lineage tracing experiments. It provides functionality for data preprocessing, tree reconstruction, simulation, visualization, and various analytical tools for working with phylogenetic trees.

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What does it do?

The Cassiopeia package offers a wide range of functionalities:

  1. Data Preprocessing: Converts raw sequencing data to formats suitable for phylogenetic analysis, including filtering, error correction, and allele calling.

  2. Tree Reconstruction: Implements various algorithms for reconstructing phylogenetic trees from mutation data, including greedy, distance-based, spectral, and integer linear programming methods.

  3. Simulation: Provides tools to generate synthetic phylogenetic trees and associated data, allowing researchers to test and validate analysis methods.

  4. Visualization: Offers both local and cloud-based options for visualizing phylogenetic trees and associated data.

  5. Analysis Tools: Includes utilities for parameter estimation, fitness calculation, evolutionary coupling analysis, and various tree metrics.

  6. Tree Comparison: Implements methods for comparing phylogenetic trees, such as Robinson-Foulds distance and triplets correct accuracy.

These functionalities enable researchers to perform end-to-end analysis of single-cell lineage tracing data, from raw sequencing results to detailed phylogenetic insights.

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Entry points

The main entry points for the Cassiopeia package are:

  1. cassiopeia.preprocess: For preprocessing raw sequencing data.
  2. cassiopeia.solver: For reconstructing phylogenetic trees from processed data.
  3. cassiopeia.simulator: For generating synthetic phylogenetic data.
  4. cassiopeia.plotting: For visualizing phylogenetic trees and associated data.
  5. cassiopeia.tools: For various analytical tools and utilities.
  6. cassiopeia.critique: For comparing and analyzing phylogenetic trees.

The __init__.py file in the root directory serves as the main interface, importing and exposing key functionalities from these submodules.

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Key Files

  1. data/CassiopeiaTree.py: Defines the core data structure for representing phylogenetic trees with associated mutation data.
  2. solver/CassiopeiaSolver.py: Provides the base class for tree reconstruction algorithms.
  3. simulator/TreeSimulator.py: Offers the base class for tree simulation models.
  4. plotting/local.py and plotting/itol_utilities.py: Implement local and cloud-based tree visualization.
  5. tools/parameter_estimators.py: Contains functions for estimating key parameters from tree data.
  6. critique/compare.py: Implements tree comparison algorithms.

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Dependencies

Cassiopeia relies on several external libraries:

  1. numpy and pandas: For data manipulation and numerical computations.
  2. networkx: For graph operations and tree manipulations.
  3. scipy: For various scientific computing tasks.
  4. ete3: For phylogenetic tree manipulation and visualization.
  5. matplotlib and plotly: For local plotting.
  6. pysam: For handling sequencing data formats.
  7. cvxpy and gurobipy: For optimization problems in some solvers.

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Configuration

Cassiopeia uses configuration files and function parameters for customization:

  1. Preprocessing pipeline: Configured using an INI-format file specifying parameters for each preprocessing step.
  2. Solvers: Customizable through parameters passed during initialization.
  3. Simulators: Configurable via parameters set during object creation.
  4. Visualization: Customizable through function parameters for both local and cloud-based plotting.

Users can adjust these configurations to adapt Cassiopeia to their specific experimental setups and analysis requirements.

In summary, Cassiopeia provides a comprehensive framework for analyzing single-cell lineage tracing data, offering tools for every step from raw data processing to advanced phylogenetic analysis and visualization.

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